One of the most commonly used open-source software is CellProfiler 5.
Various software (both commercial and open-source) have been developed 2– 4 for image and computational data analysis.
These large bioimaging datasets raised a strong need for automated and objective analysis tools 1. Recent improvements in microscopy and computational cell biology have led to an explosion of data volume, often as large as millions of images. Our analysis shows that combining local cellular features with the properties of the cell's microenvironment significantly improves the accuracy of machine learning-based phenotyping. This effect was found to be especially strong for established tissues, while it was somewhat weaker in the case of cell cultures. Our experimental data verify that the microenvironment of a cell largely determines its entity. The proposed methodology was tested for different sizes of Euclidean and nearest neighbour-based cellular environments both on tissue sections and cell cultures. Here, we present how various microenvironmental features contribute to identifying a cell and how such additional information can improve single-cell-level phenotypic image analysis. However, an important limitation of such methods is that they do not use the information gained from the cellular micro- and macroenvironment: the algorithmic decision is based solely on the local properties of the cell of interest. Due to their efficiency, machine learning-based analysis software have become essential tools to perform single-cell-level phenotypic analysis of large imaging datasets.
#Weka 3.8.1 download manual
Modern automated microscopes produce vast amounts of images routinely, making manual analysis nearly impossible. To answer major questions of cell biology, it is essential to understand cellular complexity.
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